Composite

Part:BBa_K3453124:Design

Designed by: Maeva Cherriere   Group: iGEM20_Evry_Paris-Saclay   (2020-10-04)


Rosewood DmMatK Trigger 2.1 transcription unit


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 46
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 46
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 46
    Illegal BamHI site found at 57
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 46
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 46
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T7 promoter (BBa_K2150031) + Rosewood DmMatK Trigger 2.1 (BBa_K3453024) + the strong SBa_000587 Terminator (BBa_K3453000)

Source

DNA synthesis & Assembly

References

[1] Green AA, Silver PA, Collins JJ, Yin P. Toehold switches: de-novo-designed regulators of gene expression. Cell (2014) 159, 925-939.